Tested Applications
| Positive WB detected in | human placenta tissue, PC-3 cells, SH-SY5Y cells |
| Positive IF-P detected in | mouse eye tissue |
| Positive IF/ICC detected in | SH-SY5Y cells, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
14578-1-AP targets Pikachurin in WB, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5986 Product name: Recombinant human EGFLAM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-152 aa of BC033177 Sequence: MRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDSVDGKNINTCGAK Predict reactive species |
| Full Name | EGF-like, fibronectin type III and laminin G domains |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC033177 |
| Gene Symbol | Pikachurin |
| Gene ID (NCBI) | 133584 |
| RRID | AB_2277653 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q63HQ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This antibody detects a 60-kDa band, which has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Pikachurin antibody 14578-1-AP | Download protocol |
| WB protocol for Pikachurin antibody 14578-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Novel Grm6 Variant in a no b-wave (nob) Mouse Model: Phenotype Characterization and Gene Therapy | ||
Stem Cells Dev Pikachurin Is Partially Involved in the Synaptic Connection Between Donor and Host Cells in Late-Stage rd1 Mice Following Conspecific Photoreceptor Transplantation. | ||
iScience The prion protein is required for normal responses to light stimuli by photoreceptors and bipolar cells | ||
Int Immunopharmacol EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Christoffer (Verified Customer) (07-01-2025) | This antibody works nicely on cryosections, antigen retrieval is a must (Citrate Buffer, pH 6). Does not work on paraffin-embedded tissues.
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