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  • KD/KO Validated

HJURP Polyclonal antibody

HJURP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15283-1-AP

Synonyms

FAKTS, FLEG1, hFLEG1, HJURP, Up regulated in lung cancer 9, URLC9



Tested Applications

Positive WB detected inHT-29 cells, A549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15283-1-AP targets HJURP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HJURP fusion protein Ag7402
Full Name Holliday junction recognition protein
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC001940
Gene Symbol HJURP
Gene ID (NCBI) 55355
RRIDAB_2116879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

Protocols

Product Specific Protocols
WB protocol for HJURP antibody 15283-1-APDownload protocol
IHC protocol for HJURP antibody 15283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

CENP-A Ubiquitylation Is Indispensable to Cell Viability.

Authors - Yohei Niikura
humanIHC

Front Cell Dev Biol

Comprehensive Analysis Uncovers Prognostic and Immunogenic Characteristics of Cellular Senescence for Lung Adenocarcinoma.

Authors - Weihao Lin
humanIHC

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu
humanWB

J Biol Chem

Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone HJURP.

Authors - Jianyu Wang
  • KD Validated
humanWB

Biochem Biophys Res Commun

Hypomethylation-driven overexpression of HJURP promotes progression of hepatocellular carcinoma and is associated with poor prognosis.

Authors - Ye Li
  • KD Validated

J Vis Exp

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A).

Authors - Yohei Niikura