Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 15633-1-AP
WB result of ACOT1/2 antibody (15633-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACOT1/2 transfected HEK-293 cells.
WB analysis using 15633-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT1/2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15633-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15633-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15633-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 15633-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15633-1-AP (ACOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15633-1-AP
IP result of anti-ACOT1/2 (IP:15633-1-AP, 3ug; Detection:15633-1-AP 1:500) with HepG2 cells lysate 1700ug.
IHC staining of human heart using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15633-1-AP (ACOT1/2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15633-1-AP (ACOT1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15633-1-AP (ACOT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skeletal muscle using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skeletal muscle using 15633-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15633-1-AP (ACOT2 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HepG2 using 15633-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACOT1/2 antibody (15633-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 15633-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACOT2 antibody (15633-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

ACOT1/2 Polyclonal antibody

Name: ACOT1/2 Polyclonal antibody
Brand: Proteintech
SKU: 15633-1-AP
Rating: 4.00 (1 reviews)

ACOT1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15633-1-AP

Publications(10)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ACOT2, ACOT1, acyl CoA thioesterase 2, Acyl-CoA thioesterase 2, Acyl-coenzyme A thioester hydrolase 2a

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (10)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, human brain tissue, human kidney tissue, human testis tissue, mouse kidney tissue, HepG2 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human kidney tissue, human skeletal muscle tissue, human heart tissue, human testis tissue, human liver tissue, human spleen tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IF	See 1 publications below

Product Information

15633-1-AP targets ACOT1/2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8093 Product name: Recombinant human ACOT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 222-483 aa of BC006335 Sequence: IVDMFGTGGGLLEYRASLLAGKGFAVMALAYYNYEDLPKTMETLHLEYFEEAMNYLLSHPEVKGPGVGLLGISKGGELCLSMASFLKGITAAVVINGSVANVGGTLRYKGETLPPVGVNRNRIKVTKDGYADIVDVLNSPLEGPDQKSFIPVERAESTFLFLVGQDDHNWKSEFYANEACKRLQAHGRRKPQIICYPETGHYIEPPYFPLCRASLHALVGSPIIWGGEPRAHVMAQVDAWKQLQTFFHKHLGGHEGTIPSKV Predict reactive species
Full Name	acyl-CoA thioesterase 2
Calculated Molecular Weight	483 aa, 53 kDa
Observed Molecular Weight	46-53 kDa
GenBank Accession Number	BC006335
Gene Symbol	ACOT2
Gene ID (NCBI)	10965
RRID	AB_2221535
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49753
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxidative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300). ACOT2 shares 98% homology with ACOT1, and this antibody can detect both ACOT1 and ACOT2.

Protocols

Product Specific Protocols
IF protocol for ACOT1/2 antibody 15633-1-AP	Download protocol
IHC protocol for ACOT1/2 antibody 15633-1-AP	Download protocol
IP protocol for ACOT1/2 antibody 15633-1-AP	Download protocol
WB protocol for ACOT1/2 antibody 15633-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Dev Cell Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation Authors - Wanxin Guo View Article
mouse	WB	Eur J Immunol YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice. Authors - Satabdi Nandi View Article
mouse	WB	Life Sci Alliance High levels of TFAM repress mammalian mitochondrial DNA transcription in vivo Authors - Nina A Bonekamp View Article
mouse	WB	Biomed Res Int Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression. Authors - Yuka Murata View Article
rat	WB	J Pharm Biomed Anal Application of microdialysis combined with lipidomics to analyze fatty acid metabolic changes in the disease process of rheumatoid arthritis Authors - Xiao-Man Jiang View Article
mouse	WB	Toxicol Sci Quantitative Label-Free Proteomic Analysis of Mouse Ovarian Antral Follicles Following Oral Exposure to a Human Relevant Mixture of Three Phthalates Authors - Kara L Miller View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Lana (Verified Customer) (06-21-2019)

Lines on WB:1. BioRad Precision Plus Protein standards2. Homogenate of human cortex3. Non-synaptosomal mitochondria of human cortex4. Homogenate of mouse brains5. Non-synaptosomal mitochondria of mouse brains6. Whole cell lysate of rat PC12 cells7. Mitochondria of rat C12 cellsNotes: Specific band 48 kDa in human samples only. Strong non-specific band 40 kDa in all samples types.WB protocol:SDS-PAGE: 15 ug/ul of RIPA lysates, 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1 h incubation at room temperature.