Tested Applications
| Positive WB detected in | Jurkat cells, mouse thymus tissue, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
15745-1-AP targets TAF6L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8180 Product name: Recombinant human TAF6L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC008785 Sequence: MSEREERRFVEIPRESVRLMAESTGLELSDEVAALLAEDVCYRLREATQNSSQFMKHTKRRKLTVEDFNRALRWSSVEAVCGYGSQEALPMRPAREGELYFPEDREVNLVELALATNIPKGCAETAVRVHVSYLDGKGNLAPQGSVPSAVSSLTDDLLKYYHQVTRAVLGDDPQLMKVALQDLQTNSKIGALLPYFVYVVSGVKSVSHDLEQLHRLLQVARSLFRNPHLCLGPYVRCLVGSVLYCVLEPLAASINPLNDHWTLRDGAALLLSHIFWTHGDLVSGLYQHILLSLQKILADPVRPLCCHYGAVVGLHALGWKAVERVLYPHLSTYWTNLQAVLDDYSVSNAQV Predict reactive species |
| Full Name | TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa |
| Calculated Molecular Weight | 622 aa, 68 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC008785 |
| Gene Symbol | TAF6L |
| Gene ID (NCBI) | 10629 |
| RRID | AB_2271394 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6J9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TAF6L (TBP-associated factor 6-like protein, also known as PAF65α) is a core component of the PCAF histone acetyltransferase complex and the STAGA transcriptional coactivation complex. This protein contains a characteristic histone folding domain (HFD) and is primarily located in the cell nucleus. As an epigenetic regulator, TAF6L works synergistically with TAF5L to promote the transcriptional elongation of RNA polymerase II by mediating H3K9ac deposition and MYC recruitment, thereby regulating embryonic stem cell pluripotency and somatic cell reprogramming.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF6L antibody 15745-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
