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SMYD1-Specific Polyclonal antibody

SMYD1-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16151-1-AP

Synonyms

BOP, SMYD1, SMYD1-Specific, ZMYND18, ZMYND22

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    WB analysis of mouse heart using 16151-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    WB analysis of mouse skeletal muscle using 16151-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IP experiment of mouse heart using 16151-1-AP

    IP result of anti-SMYD1-Specific (IP:16151-1-AP, 4ug; Detection:16151-1-AP 1:500) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human testis using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human testis using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of mouse heart using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of mouse heart using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human heart using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human heart using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human kidney using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human kidney using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human placenta using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human placenta using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human skin using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human skin using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human liver using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human liver using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human spleen using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human spleen using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human lung using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human lung using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human ovary using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SMYD1-Specific Polyclonal antibody (16151-1-AP)

    IHC staining of human ovary using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human liver tissue, human spleen tissue, human lung tissue, human ovary tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16151-1-AP targets SMYD1-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SET and MYND domain containing 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberNM_198274
Gene Symbol SMYD1
Gene ID (NCBI) 150572
RRIDAB_2239747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SMYD1-Specific antibody 16151-1-APDownload protocol
IHC protocol for SMYD1-Specific antibody 16151-1-APDownload protocol
IP protocol for SMYD1-Specific antibody 16151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Toxicol Lett

Down-regulated Smyd1 participated in the inhibition of myoblast differentiation induced by cigarette smoke extract

Authors - Fang Li
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Deyong (Verified Customer) (03-11-2022)

SMYD1 antibody worked very well in human differentiated cardiomyocyte.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: Human cardiomyocytes
FH

Eric (Verified Customer) (10-15-2018)

Very low detection of SMYD1: 45 ug protein, overnight incubation with primary antibody at 4 oC, 2h incubation with secondary at room temperature.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: C2C12/mouse heart