Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 16242-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16242-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16242-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 16242-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16242-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16242-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 16242-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 16242-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 16242-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 16242-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16242-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SDSL antibody (16242-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SDSL Polyclonal antibody

Name: SDSL Polyclonal antibody
Brand: Proteintech
SKU: 16242-1-AP

SDSL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16242-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:4.3.1.17, EC:4.3.1.19, L serine deaminase, L threonine dehydratase, L-serine deaminase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, human colon tissue, human liver tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected in	human liver tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16242-1-AP targets SDSL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9114 Product name: Recombinant human SDSL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC009849 Sequence: MDGPVAEHAKQEPFHVVTPLLESWALSQVAGMPVFLKCENVQPSGSFKIRGIGHFCQEMAKKGCRHLVCSSGGNAGIAAAYAARKLGIPATIVLPESTSLQVVQRLQGEGAEVQLTGKVWDEANLRAQELAKRDGWENVPPFDHPLIWKGHASLVQELKAVLRTPPG Predict reactive species
Full Name	serine dehydratase-like
Calculated Molecular Weight	329 aa, 35 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	BC009849
Gene Symbol	SDSL
Gene ID (NCBI)	113675
RRID	AB_2254163
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96GA7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDSL (full name: Serine Dehydratase-Like) is a pyridoxal phosphate (PLP)-dependent enzyme encoded by human SDSL gene, which is mainly located in cytoplasm. Biochemical activity: It is a multifunctional enzyme with weak L- serine ammonia-lyase and L- threonine dehydratase activities, and participates in the process of amino acid catabolism (catalyzing serine and threonine to be converted into pyruvate, α-ketobutyric acid and ammonia respectively). Studies have shown that compared with the homologous enzyme SDS in the liver, the activity of SDSL in human cancer cells is lower. Clinical and research significance: This protein plays a certain role in signal transduction and metabolic regulation. Abnormal expression or variation at the gene level is related to specific genetic diseases (such as some thyroid dysfunction) and cancer mechanism research. Homodimer can be formed.

Protocols

Product Specific Protocols
IF protocol for SDSL antibody 16242-1-AP	Download protocol
IHC protocol for SDSL antibody 16242-1-AP	Download protocol
WB protocol for SDSL antibody 16242-1-AP	Download protocol