TST Polyclonal antibody

TST Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16311-1-AP

Synonyms

RDS, Rhodanese, Thiosulfate sulfurtransferase, TST



Tested Applications

Positive WB detected inHEK-293 cells, liver tissue, human kidney tissue, HeLa cells, HepG2 cells, mouse kidney, rat kidney
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 8 publications below

Product Information

16311-1-AP targets TST in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TST fusion protein Ag9346
Full Name thiosulfate sulfurtransferase (rhodanese)
Calculated Molecular Weight 297 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC010148
Gene Symbol TST
Gene ID (NCBI) 7263
RRIDAB_2211217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.

Protocols

Product Specific Protocols
WB protocol for TST antibody 16311-1-APDownload protocol
IHC protocol for TST antibody 16311-1-APDownload protocol
IP protocol for TST antibody 16311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Mol Med

Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway.

Authors - Marcello Ziosi
mouseWB

EMBO Mol Med

CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.

Authors - Marta Luna-Sánchez
mouseWB

Antioxid Redox Signal

The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS(-) Production and Evidence of Pancreatic and Lung Toxicity.

Authors - Roman Heather B HB
mouse,humanWB

Hum Mol Genet

Coenzyme Q10 modulates sulfide metabolism and links the mitochondrial respiratory chain to pathways associated to one carbon metabolism.

Authors - Pilar González-García
mouseWB

Biochem Pharmacol

Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis.

Authors - Katalin Módis
mouseWB

Amino Acids

Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate.

Authors - Halina Jurkowska