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  • Featured Product
  • KD/KO Validated

ADO Polyclonal antibody

ADO Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16479-1-AP

Synonyms

2 aminoethanethiol dioxygenase, ADO, C10orf22, Cysteamine dioxygenase

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ADO Polyclonal antibody (16479-1-AP)

    WB analysis of human brain using 16479-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ADO Polyclonal antibody (16479-1-AP)

    WB analysis of mouse spleen using 16479-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using ADO Polyclonal antibody (16479-1-AP)

    IHC staining of human brain using 16479-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ADO Polyclonal antibody (16479-1-AP)

    IHC staining of human brain using 16479-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue, mouse spleen tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16479-1-AP targets ADO in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADO fusion protein Ag9598
Full Name 2-aminoethanethiol (cysteamine) dioxygenase
Calculated Molecular Weight 270 aa, 30 kDa
Observed Molecular Weight 28 kDa-30 kDa
GenBank Accession NumberBC018660
Gene Symbol ADO
Gene ID (NCBI) 84890
RRIDAB_2222204
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADO antibody 16479-1-APDownload protocol
IHC protocol for ADO antibody 16479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Discov

ADO/hypotaurine: a novel metabolic pathway contributing to glioblastoma development.

Authors - Dachuan Shen
  • KO Validated
View Article