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GRB10 Polyclonal antibody

GRB10 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16622-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GRB10 Polyclonal antibody (16622-1-AP)

    WB analysis of HepG2 using 16622-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16622-1-AP (GRB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GRB10 Polyclonal antibody (16622-1-AP)

    WB analysis of HepG2 using 16622-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16622-1-AP (GRB10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRB10 Polyclonal antibody (16622-1-AP)

    IF Staining of HepG2 using 16622-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16622-1-AP (GRB10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16622-1-AP targets GRB10 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRB10 fusion protein Ag9921
Full Name growth factor receptor-bound protein 10
Calculated Molecular Weight536aa,61 kDa; 594aa,67 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC024285
Gene Symbol GRB10
Gene ID (NCBI) 2887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the ins (INSR) and ins-like growth factor (IGF1R) receptors. It may play a role in mediating ins-stimulated ubiquitination of INSR, leading to proteasomal degradation.

Protocols

Product Specific Protocols
WB protocol for GRB10 antibody 16622-1-APDownload protocol
IF protocol for GRB10 antibody 16622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols