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Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, human brain tissue, mouse colon tissue, mouse lung tissue, rat colon tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 17 publications below |
| IP | See 3 publications below |
| CoIP | See 2 publications below |
Product Information
16636-1-AP targets CCDC22 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9980 Product name: Recombinant human CCDC22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 283-628 aa of BC000972 Sequence: AKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA Predict reactive species |
| Full Name | coiled-coil domain containing 22 |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC000972 |
| Gene Symbol | CCDC22 |
| Gene ID (NCBI) | 28952 |
| RRID | AB_2072065 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60826 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CCDC22 antibody 16636-1-AP | Download protocol |
| WB protocol for CCDC22 antibody 16636-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. | ||
Nat Commun A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. | ||
Nat Commun CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. | ||
J Cell Biol Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling. | ||
Mol Metab Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. |
