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Tested Applications
| Positive WB detected in | mouse ovary tissue, HeLa cells, mouse lung tissue, mouse testis tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | mouse ovary tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IF | See 11 publications below |
| IP | See 2 publications below |
Product Information
17533-1-AP targets DNAI2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11585 Product name: Recombinant human DNAI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 239-593 aa of BC039582 Sequence: GQIACWDTRKGSLVAELSTIESSHRDPVYGTIWLQSKTGTECFSASTDGQVMWWDIRKMSEPTEVVILDITKKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAKTSAEKIVCTFPGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLTDAAWSPVRPTVFFTTRMDGTLDIWDFMFEQCDPTLSLKDNGCLIACGSQLGTTTLLEVSPGLSTLQRNEKNVASSMFERETRREKILEARHREMRLKEKGKAEGRDEEQTDEELAVDLEALVSKAEEEFFDIIFTELKKKEADAIKLTPVPQQPSPEEDQVVEEGEEAAGEEGDEEVEEDLA Predict reactive species |
| Full Name | dynein, axonemal, intermediate chain 2 |
| Calculated Molecular Weight | 593 aa, 67 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC039582 |
| Gene Symbol | DNAI2 |
| Gene ID (NCBI) | 64446 |
| RRID | AB_2096670 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZS0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAI2 antibody 17533-1-AP | Download protocol |
| IHC protocol for DNAI2 antibody 17533-1-AP | Download protocol |
| IP protocol for DNAI2 antibody 17533-1-AP | Download protocol |
| WB protocol for DNAI2 antibody 17533-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Mol Cell Biol Production of transgenic mice by random recombination of targeted genes in female germline stem cells. | ||
Front Cell Dev Biol PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia | ||
Development CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility | ||
Exp Anim The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum. | ||
Cytoskeleton (Hoboken) Axonemal Dynein Visualized in Primary Cilia via Expansion Microscopy |
