Chk1 Monoclonal antibody
Chk1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 65016-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K562 cells, K-562 |
Positive IHC detected in | Prostate cancer tissue, human prostate cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
65016-1-Ig targets Chk1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant Protein |
Full Name | CHK1 checkpoint homolog (S. pombe) |
Calculated Molecular Weight | 54 kDa |
Observed Molecular Weight | 54 kDa |
GenBank Accession Number | BC004202 |
Gene Symbol | CHEK1 |
Gene ID (NCBI) | 1111 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at 2-8°C. 20ul sizes contain 0.1% BSA. |
Background Information
In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. Chk1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases.
Publications
Species | Application | Title |
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