Tested Applications
| Positive WB detected in | human plasma tissue, BxPC-3 cells, HepG2 cells, human plasma, MCF-7 cells, mouse kidney tissue |
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14181-1-AP targets Alpha 1B-Glycoprotein/A1BG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5414 Product name: Recombinant human A1BG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-373 aa of BC035719 Sequence: MAPVSWITPGLKTTAVCRGVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVTIEELAAPPPPVLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCRYRLHDNQNGWSGDSAPVELILSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQSPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPPPRPQLRATWSGAVLAGRDAVLRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVELLVAES Predict reactive species |
| Full Name | alpha-1-B glycoprotein |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 65-75 kDa |
| GenBank Accession Number | BC035719 |
| Gene Symbol | A1BG |
| Gene ID (NCBI) | 1 |
| RRID | AB_10640441 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04217 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Alpha 1B-Glycoprotein/A1BG antibody 14181-1-AP | Download protocol |
| IHC protocol for Alpha 1B-Glycoprotein/A1BG antibody 14181-1-AP | Download protocol |
| WB protocol for Alpha 1B-Glycoprotein/A1BG antibody 14181-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The A1BG Polyclonal Antibody (Cat# 14181-1-AP) has 1 citation to date. It appears in Advanced Science (2025), where it was used to study how adipocytes promote cisplatin resistance in osteosarcoma through A1BG secretion and the NAMPT/PARP1 DNA repair axis. The research falls within the fields of cancer biology, drug resistance mechanisms, and DNA repair.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Adipocytes Promote Cisplatin Resistance through Secreting A1BG and Regulating NAMPT/PARP1 Axis-Mediated DNA Repair in Osteosarcoma |
Reviews
What customers say
One reviewer rated this antibody 4/5 stars for Western Blot at a 1:2000 dilution. They reported good performance with human tissues, including human plasma and MCF7 cells. However, the antibody did not recognize A1BG of mouse origin when tested on mouse plasma and mouse kidney. Overall, it is recommended for human samples but not suitable for cross-reactivity with mouse A1BG.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (12-04-2025) | Good antibody for WB with human tissues. It does not recognize A1BG of mouse origin.
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