Filter: AllWBIHCIF/ICC

WB analysis of RAW 264.7 using 12392-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12392-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 12392-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 12392-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12392-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12392-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12392-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12392-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using A4GALT antibody (12392-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

A4GALT Polyclonal antibody

Name: A4GALT Polyclonal antibody
Brand: Proteintech
SKU: 12392-1-AP

A4GALT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12392-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.4.1.228, Alpha4Gal-T1, Alpha-1,4-N-acetylglucosaminyltransferase, Alpha-1,4-galactosyltransferase, A4GALT1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	RAW 264.7 cells, K-562 cells, Raji cells
Positive IHC detected in	human cervical cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12392-1-AP targets A4GALT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3069 Product name: Recombinant human A4GALT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-353 aa of BC017068 Sequence: HVVGEPKEKGQLYNLPAEIPCPTLTPPTPPSHGPTPGNIFFLETSDRTNPNFLFMCSVESAARTHPESHVLVLMKGLPGGNASLPRHLGISLLSCFPNVQMLPLDLRELFRDTPLADWYAAVQGRWEPYLLPVLSDASRIALMWKFGGIYLDTDFIVLKNLRNLTNVLGTQSRYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAMKMYL Predict reactive species
Full Name	alpha 1,4-galactosyltransferase
Calculated Molecular Weight	353 aa, 41 kDa
Observed Molecular Weight	50-52 kDa
GenBank Accession Number	BC017068
Gene Symbol	A4GALT
Gene ID (NCBI)	53947
RRID	AB_2877853
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NPC4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for A4GALT antibody 12392-1-AP	Download protocol
IHC protocol for A4GALT antibody 12392-1-AP	Download protocol
WB protocol for A4GALT antibody 12392-1-AP	Download protocol