AATF Antibody
Rabbit Polyclonal
Catalog number: 10282-1-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse brain tissue |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:200-1:2000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10282-1-AP(AATF Antibody) at dilution of 1:800
Immunofluorescent analysis of Hela cells, using AATF antibody 10282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | AATF fusion protein ag0195 | Full name | apoptosis antagonizing transcription factor |
|
Calculated molecular weight |
63kd |
Observed molecular
weight |
63kd |
|
GenBank accession number |
BC000591 | Gene ID (NCBI) | 26574 |
| Gene symbol | AATF | Synonyms | CHE-1; CHE1; DED |
Background
Apoptosis antagonizing transcription factor (AATF) is a nuclear phosphoprotein of 523 amino acids and contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis. AATF also binds Rb and the core of pol II, and may be part of transcription regulatory complex. |
