Tested Applications
| Positive WB detected in | human brain tissue, HeLa cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11159-1-AP targets ABCD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1635 Product name: Recombinant human ABCD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 334-507 aa of BC025358 Sequence: LMKYVWSASGLLMVAVPIITATGYSESDAEAVKKAALEKKEEELVSERTEAFTIARNLLTAAADAIERIMSSYKEVTELAGYTARVHEMFQVFEDVQRCHFKRPRELEDAQAGSGTIGRSGVRVEGPLKIRGQVVDVEQGIICENIPIVTPSGEVVVASLNIRVEEGMHLLITG Predict reactive species |
| Full Name | ATP-binding cassette, sub-family D (ALD), member 1 |
| Calculated Molecular Weight | 745 aa, 83 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC025358 |
| Gene Symbol | ABCD1 |
| Gene ID (NCBI) | 215 |
| RRID | AB_2219775 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P33897 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ABCD1 antibody 11159-1-AP | Download protocol |
| IHC protocol for ABCD1 antibody 11159-1-AP | Download protocol |
| IP protocol for ABCD1 antibody 11159-1-AP | Download protocol |
| WB protocol for ABCD1 antibody 11159-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ABCD1 Polyclonal Antibody (Cat# 11159-1-AP) has 3 total citations. These appear in Cell Biology and Toxicology, Cancers (Basel), and Journal of Biomedical Research. The associated research fields span oral cancer stem cell biology, prostate cancer proliferation with peroxisomal alterations, and PEX3-dependent peroxisome roles in spermiogenesis. Applications cited include Western blotting and immunofluorescence in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Biol Toxicol CD44-targeted therapy using mP6/Rg3 micelles inhibits oral cancer stem cell proliferation and migration. | ||
Cancers (Basel) Prostate Cancer Proliferation Is Affected by the Subcellular Localization of MCT2 and Accompanied by Significant Peroxisomal Alterations. | ||
J Biomed Res Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis |
