Filter: AllWBIHCIF/ICC

WB analysis of L02 using 20952-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 20952-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human prostate hyperplasia using 20952-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate hyperplasia using 20952-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 20952-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 20952-1-AP (ABHD14B antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 20952-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 20952-1-AP (ABHD14B antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 20952-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ABHD14B antibody (20952-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ABHD14B Polyclonal antibody

Name: ABHD14B Polyclonal antibody
Brand: Proteintech
SKU: 20952-1-AP

ABHD14B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20952-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Abhydrolase domain-containing protein 14B, Alpha/beta hydrolase domain-containing protein 14B, CCG1-interacting factor B, CIB, EC:2.3.1.-

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	L02 cells, mouse small intestine tissue
Positive IHC detected in	human prostate hyperplasia tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20952-1-AP targets ABHD14B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15097 Product name: Recombinant human ABHD14B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-210 aa of BC007234 Sequence: MAASVEQREGTIQVQGQALFFREALPGSGQARFSVLLLHGIRFSSETWQNLGTLHRLAQAGYRAVAIDLPGLGHSKEAAAPAPIGELAPGSFLAAVVDALELGPPVVISPSLSGMYSLPFLTAPGSQLPGFVPVAPICTDKINAANYASVKTPALIVYGDQDPMGQTSFEHLKQLPNHRVLIMKGAGHPCYLDKPEEWHTGLLDFLQGLQ Predict reactive species
Full Name	abhydrolase domain containing 14B
Calculated Molecular Weight	210 aa, 22 kDa
Observed Molecular Weight	22-25 kDa
GenBank Accession Number	BC007234
Gene Symbol	ABHD14B
Gene ID (NCBI)	84836
RRID	AB_10733758
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96IU4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABHD14B possessed the canonical ABHD fold, an invariant catalytic triad (Ser-His-Asp), and based on its protein sequence, was subsequently categorized as an outlying member of the metabolic serine hydrolase family. ABHD14B was able to transfer an acetyl group from post translationally modified protein lysine residue to coenzyme-A (CoA), thus making the biologically important acetyl-CoA.

Protocols

Product Specific Protocols
IF protocol for ABHD14B antibody 20952-1-AP	Download protocol
IHC protocol for ABHD14B antibody 20952-1-AP	Download protocol
WB protocol for ABHD14B antibody 20952-1-AP	Download protocol