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ABHD5 Polyclonal antibody

ABHD5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12201-1-AP

Synonyms

ABHD5, CDS, CGI58, IECN2, NCIE2



Tested Applications

Positive WB detected inA431 cells, A549 cells, HCT 116 cells, HeLa cells, HepG2 cells, LNCaP cells, NIH/3T3 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skeletal muscle tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12201-1-AP targets ABHD5 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD5 fusion protein Ag2840
Full Name abhydrolase domain containing 5
Calculated Molecular Weight 349 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC021958
Gene Symbol ABHD5
Gene ID (NCBI) 51099
RRIDAB_2220710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

Protocols

Product Specific Protocols
WB protocol for ABHD5 antibody 12201-1-APDownload protocol
IHC protocol for ABHD5 antibody 12201-1-APDownload protocol
IF protocol for ABHD5 antibody 12201-1-APDownload protocol
IP protocol for ABHD5 antibody 12201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.

Authors - Yunfan Yang
mouseWB,CoIP

Cell Death Differ

Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation.

Authors - Yi Wu
humanWB

Proc Natl Acad Sci U S A

Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover.

Authors - Olzmann James A JA
mouseWB,IP,IF

Proc Natl Acad Sci U S A

Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.

Authors - Satish Patel
humanWB

J Cell Biol

ORP5 localizes to ER-lipid droplet contacts and regulates the level of PI(4)P on lipid droplets.

Authors - Ximing Du
humanWB

Elife

Inhibition of intracellular lipolysis promotes human cancer cell adaptation to hypoxia.

Authors - Xiaodong Zhang