ABHD5 Antibody
Rabbit Polyclonal
Catalog number: 12201-1-AP
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Applications
| Tested applications | ELISA, WB, IF |
| Cited applications | IF, WB |
| Species specificity | human; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | Jurkat cells, human brain tissue, human cerebellum tissue |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:200-1:2000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 12201-1-AP(ABHD5 Antibody) at dilution of 1:500
Immunofluorescent analysis of MCF-7 cells, using ABHD5 antibody 12201-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | ABHD5 fusion protein ag2840 | Full name | abhydrolase domain containing 5 |
|
Calculated molecular weight |
349aa,39kd |
Observed molecular
weight |
39 kDa |
|
GenBank accession number |
BC021958 | Gene ID (NCBI) | 51099 |
| Gene symbol | ABHD5 | Synonyms | CDS; CGI58; IECN2; MGC8731; NCIE2 |
Background
ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS). |
