Filter: AllWBIHC

WB analysis using 14890-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14890-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14890-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14890-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14890-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 14890-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14890-1-AP (ABI2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).

ABI2 Polyclonal antibody

ABI2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14890-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Synonyms

Abelson interactor 2, Abi-2, Abl binding protein 3, Abl-binding protein 3, AblBP3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse heart tissue, mouse uterus tissue, mouse testis tissue, mouse brain tissue, HeLa cells, K-562 cells, SH-SY5Y cells
Positive IHC detected in	human brain tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:6000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
CoIP	See 1 publications below

Product Information

14890-1-AP targets ABI2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6679 Product name: Recombinant human ABI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC001439 Sequence: MAELQMLLEEEIPGGRRALFDSYTNLERVADYCENNYIQSADKQRALEETKAYTTQSLASVAYLINTLANNVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANLERPVRYIRKPIDYTILDDIGHGVKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPSPTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSV Predict reactive species
Full Name	abl interactor 2
Calculated Molecular Weight	56 kDa
Observed Molecular Weight	52-56 kDa
GenBank Accession Number	BC001439
Gene Symbol	ABI2
Gene ID (NCBI)	10152
RRID	AB_2288997
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NYB9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ABI2 antibody 14890-1-AP	Download protocol
WB protocol for ABI2 antibody 14890-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Nat Commun GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis Authors - Qiang Zhu View Article
mouse,human	WB	Nat Commun hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS) Authors - Hannah E Salapa View Article
human	WB	Nat Cell Biol Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis Authors - Xiaoguang Liu View Article