STARS/ABRA Polyclonal antibody
STARS/ABRA Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 22673-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, human heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 22673-1-AP is STARS/ABRA Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STARS/ABRA fusion protein Ag18456 |
| Full Name | actin-binding Rho activating protein |
| Calculated Molecular Weight | 381 aa, 43 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC105103 |
| Gene Symbol | ABRA |
| Gene ID (NCBI) | 137735 |
| RRID | AB_11182712 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STARS/ABRA antibody 22673-1-AP | Download protocol |
| IHC protocol for STARS/ABRA antibody 22673-1-AP | Download protocol |
| IF protocol for STARS/ABRA antibody 22673-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Development HAND2-mediated epithelial maintenance and integrity in cardiac outflow tract morphogenesis. | ||
Behav Brain Res Changes in mRNA and miRNA expression in the prelimbic cortex related to depression-like syndrome induced by chronic social defeat stress in mice | ||
Am J Pathol Different mouse models of nemaline myopathy harboring Acta1 mutations display differing abnormalities related to mitochondrial biology |
