• Featured Product
  • KD/KO Validated

ACAA2 Polyclonal antibody

ACAA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11111-1-AP

Synonyms

ACAA2, Acetyl CoA acyltransferase, Beta ketothiolase, DSAEC, T1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, mouse liver tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11111-1-AP targets ACAA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAA2 fusion protein Ag1609
Full Name acetyl-Coenzyme A acyltransferase 2
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC001918
Gene Symbol ACAA2
Gene ID (NCBI) 10449
RRIDAB_2219389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACAA2 antibody 11111-1-APDownload protocol
IHC protocol for ACAA2 antibody 11111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein.

Authors - Yanxiao Zhang
ratWB

EBioMedicine

PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.

Authors - Zhongshi Lyu
ratWB,IHC

PLoS One

Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.

Authors - Baandrup Jonas D JD
sheepWB

Front Genet

Identification of miRNA in Sheep Intramuscular Fat and the Role of miR-193a-5p in Proliferation and Differentiation of 3T3-L1.

Authors - Fuhui Han
ratWB

Gerontology

Dietary Restriction Increases Protein Acetylation in the Livers of Aged Rats.

Authors - Nakamura Akihiro A
humanWB

J Biol Chem

CLPX regulates mitochondrial fatty acid β-oxidation in liver cells

Authors - Ko Suzuki