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ACAA2 Polyclonal antibody

ACAA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11111-1-AP

Publications (21)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

3-ketoacyl-CoA thiolase, mitochondrial, Acetyl CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl-CoA acyltransferase, Acyl-CoA hydrolase, mitochondrial

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 11111-1-AP
WB result of ACAA2 antibody (11111-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAA2 transfected HEK-293 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 11111-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11111-1-AP (ACAA2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 11111-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11111-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAA2 antibody (11111-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected in	human lung cancer tissue, human colon tissue, human liver tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11111-1-AP targets ACAA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1609 Product name: Recombinant human ACAA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 98-397 aa of BC001918 Sequence: SIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMGIGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA Predict reactive species
Full Name	acetyl-Coenzyme A acyltransferase 2
Calculated Molecular Weight	42 kDa
Observed Molecular Weight	42 kDa
GenBank Accession Number	BC001918
Gene Symbol	ACAA2
Gene ID (NCBI)	10449
RRID	AB_2219389
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42765
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ACAA2 antibody 11111-1-AP	Download protocol
IHC protocol for ACAA2 antibody 11111-1-AP	Download protocol
WB protocol for ACAA2 antibody 11111-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The ACAA2 Polyclonal antibody (Cat# 11111-1-AP) has been cited in 21 publications across journals including Neuron, Nature Chemical Biology, EBioMedicine, Oncogene, iScience, FASEB Journal, Journal of Biological Chemistry, Free Radical Biology and Medicine, Molecular Carcinogenesis, and PLoS ONE. Associated research fields encompass fatty acid oxidation and metabolic homeostasis, oncology, renal disease, diabetes, mitochondrial biology, neurology, and endocrinology.

Species	Application	Title
		Neuron Alpha-synuclein mutations mislocalize cytoplasmic p300 compromising autophagy, which is rescued by ACLY inhibition Authors - Sung Min Son View Article
human	WB	Nat Chem Biol The molecular glue CLEO4-88 inhibits the ACAA1 thiolase by induced binding to GID4. Authors - Chetan K Chana View Article
mouse	WB	J Pharm Anal Signatures of proteomics and glycoproteomics revealed liraglutide ameliorates MASLD by regulating specific metabolic homeostasis in mice. Authors - Yuxuan Chen View Article
rat	WB	EBioMedicine PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules. Authors - Zhongshi Lyu View Article
rat	WB	Phytother Res Icariside II prevents kidney fibrosis development in chronic kidney disease by promoting fatty acid oxidation Authors - Meng Wang View Article
mouse	WB	Free Radic Biol Med Low-intensity pulsed ultrasound ameliorates pancreatic injury and glucose metabolism in type 2 diabetic mice: regulatory role of the oxidative phosphorylation pathway. Authors - Chen Ni View Article