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ACADL Polyclonal antibody

ACADL Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17442-1-AP

Synonyms

ACAD4, ACADL, LCAD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis using 17442-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of HeLa using 17442-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of HepG2 using 17442-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of HeLa using 17442-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of HeLa using 17442-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of mouse kidney using 17442-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of mouse kidney using 17442-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of mouse kidney using 17442-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of rat kidney using 17442-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of NIH/3T3 using 17442-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of HEK-293 using 17442-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ACADL Polyclonal antibody (17442-1-AP)

    WB analysis of HEK-293 using 17442-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACADL Polyclonal antibody (17442-1-AP)

    IHC staining of human liver cancer using 17442-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACADL Polyclonal antibody (17442-1-AP)

    IHC staining of human liver cancer using 17442-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ACADL Polyclonal antibody (17442-1-AP)

    IHC staining of human liver using 17442-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACADL Polyclonal antibody (17442-1-AP)

    IHC staining of human liver using 17442-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inmouse kidney tissue, HepG2 cells, rat kidney tissue, HeLa cells, HEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

17442-1-AP targets ACADL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACADL fusion protein Ag11530
Full Name acyl-Coenzyme A dehydrogenase, long chain
Calculated Molecular Weight 430 aa, 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC039063
Gene Symbol ACADL
Gene ID (NCBI) 33
RRIDAB_2219662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).

Protocols

Product Specific Protocols
WB protocol for ACADL antibody 17442-1-APDownload protocol
IHC protocol for ACADL antibody 17442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients.

Authors - Ya-Kun Zhang
View Article
ratWB

Front Pharmacol

Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease

Authors - Xinhui Liu
View Article
mouseWB

Front Pharmacol

The serum acylcarnitines profile in epileptic children treated with valproic acid and the protective roles of peroxisome proliferator-activated receptor a activation in valproic acid-induced liver injury

Authors - Yiyi Ma
View Article
human,mouseWB

Diabetologia

Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation

Authors - Shao-Wen Weng
View Article