Tested Applications
| Positive WB detected in | HL-60 cells, Jurkat cells |
| Positive IHC detected in | human lung squamous cell cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20135-1-AP targets ACAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14068 Product name: Recombinant human ACAP1 protein Source: e coli.-derived, PKG Tag: GST Domain: 20-251 aa of BC018543 Sequence: SIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELG Predict reactive species |
| Full Name | ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 |
| Calculated Molecular Weight | 740 aa, 82 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC018543 |
| Gene Symbol | ACAP1 |
| Gene ID (NCBI) | 9744 |
| RRID | AB_10696311 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15027 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACAP1 antibody 20135-1-AP | Download protocol |
| IHC protocol for ACAP1 antibody 20135-1-AP | Download protocol |
| WB protocol for ACAP1 antibody 20135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACAP1 Polyclonal antibody (Cat# 20135-1-AP) has 3 citations published in Cancer Letters, Computational and Structural Biotechnology Journal, and Methods in Cell Biology. The associated research fields include esophageal squamous cell carcinoma metastasis and chemoresistance, lung adenocarcinoma prognostic biomarkers and tumor immunity, and endocytic recycling mechanisms. All citations used human samples with WB as the primary application.
| Species | Application | Title |
|---|---|---|
Cancer Lett CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma. | ||
Comput Struct Biotechnol J Integrated analysis and validation reveal ACAP1 as a novel prognostic biomarker associated with tumor immunity in lung adenocarcinoma | ||
