ACBD4 Polyclonal antibody

ACBD4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 20941-1-AP

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

HMFT0700, Acyl-CoA-binding domain-containing protein 4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIF/ICC

WB analysis of human kidney using 20941-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 20941-1-AP (ACBD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 20941-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ACBD4 antibody (20941-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for ACBD4 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	human kidney tissue
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20941-1-AP targets ACBD4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15045 Product name: Recombinant human ACBD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 73-305 aa of BC029164 Sequence: WNSLGKMSREEAMSAYITEMKLVAQKVIDTVPLGEVAEDMFGYFEPLYQVIPDMPRPPETFLRRVTGWKEQVVNGDVGAVSEPPCLPKEPAPPSPESHSPRDLDSEVFCDSLEQLEPELVWTEQRAASGGKRDPRNSPVPPTKKEGLRGSPPGPQELDVWLLGTVRALQESMQEVQARVQSLESMPRPPEQRPQPRPSARPWPLGLPGPALLFFLLWPFVVQWLFRMFRTQKR Predict reactive species
Full Name	acyl-Coenzyme A binding domain containing 4
Calculated Molecular Weight	341 aa, 38 kDa
Observed Molecular Weight	30-38 kDa
GenBank Accession Number	BC029164
Gene Symbol	ACBD4
Gene ID (NCBI)	79777
RRID	AB_2878769
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NC06
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ACBD4 antibody 20941-1-AP	Download protocol
WB protocol for ACBD4 antibody 20941-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ACBD4 Polyclonal antibody (Cat# 20941-1-AP) has 1 citation. It was cited in Nature Structural & Molecular Biology (2021), in a study investigating how TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. The research field encompasses neurodegenerative disease (ALS), oxidative stress, and mitochondrial biology. The antibody was used for Western blot in mouse samples.

Species	Application	Title
mouse	WB	Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. Authors - Xinxin Zuo View Article