Tested Applications
| Positive WB detected in | Jurkat cells, SiHa cells, THP-1 cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25849-1-AP targets ACD in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23021 Product name: Recombinant human ACD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC016904 Sequence: MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRAGRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWAPAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLTL Predict reactive species |
| Full Name | adrenocortical dysplasia homolog (mouse) |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 57-60 kDa |
| GenBank Accession Number | BC016904 |
| Gene Symbol | ACD |
| Gene ID (NCBI) | 65057 |
| RRID | AB_2880268 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ACD antibody 25849-1-AP | Download protocol |
| WB protocol for ACD antibody 25849-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACD polyclonal antibody (Cat# 25849-1-AP) has 4 citations published in Science Advances (2), Biomaterials (1), and Journal of Cancer (1). The research fields span telomere homeostasis and m6A methylation in cancer, telomeric nucleosome recognition by TRF1, peripheral nerve regeneration via electroactive conduits, and cell growth-related biomarkers for oral squamous cell carcinoma. Applications cited include IP, WB, and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Sci Adv Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A). | ||
Biomaterials Hydrogen-releasing electroactive nerve guidance conduits promote peripheral nerve regeneration by remodeling the microenvironment | ||
J Cancer Development and Validation of a Nomogram based on cell growth-related Biomarkers for Oral Squamous Cell Carcinoma. |
