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ACOT12 Polyclonal antibody

ACOT12 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17871-1-AP

Synonyms

ACOT12, acyl CoA thioesterase 12, Cach, CACH 1, CACH1, hCACH 1, STARD15, THEAL

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using ACOT12 Polyclonal antibody (17871-1-AP)

    WB analysis of COLO 320 using 17871-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17871-1-AP (ACOT12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACOT12 Polyclonal antibody (17871-1-AP)

    IHC staining of human liver cancer using 17871-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17871-1-AP (ACOT12 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACOT12 Polyclonal antibody (17871-1-AP)

    IHC staining of human liver cancer using 17871-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17871-1-AP (ACOT12 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ACOT12 Polyclonal antibody (17871-1-AP)

    IHC staining of human colon cancer using 17871-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17871-1-AP (ACOT12 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ACOT12 Polyclonal antibody (17871-1-AP)

    IHC staining of human colon cancer using 17871-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17871-1-AP (ACOT12 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using ACOT12 Polyclonal antibody (17871-1-AP)

    IF Staining of COLO 320 using 17871-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 17871-1-AP (ACOT12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17871-1-AP is ACOT12 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT12 fusion protein Ag12355
Full Name acyl-CoA thioesterase 12
Calculated Molecular Weight 555 aa, 62 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC075011
Gene Symbol ACOT12
Gene ID (NCBI) 134526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.