Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15901-1-AP targets ACOT9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8730 Product name: Recombinant human ACOT9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-212 aa of BC012573 Sequence: MRRAALRLCALGKGQLTPGRGLTQGPQNPKKQGIFHIHEVRDKLREIVGASTNWRDHVKAMEERKLLHSFLAKSQDGLPPRRMKDSYIEVLLPLGSEPELREKYLTVQNTVRFGRILEDLDSLGVLICYMHNKIHSAKMSPLSIVTALVDKIDMCKKSLSPEQDIKFSGHVSWVGKTSMEVKMQMFQLHGDEFCPVLDATFVMVARDSENKG Predict reactive species |
| Full Name | acyl-CoA thioesterase 9 |
| Calculated Molecular Weight | 24 kDa, 49 kDa |
| Observed Molecular Weight | 43-50 kDa |
| GenBank Accession Number | BC012573 |
| Gene Symbol | ACOT9 |
| Gene ID (NCBI) | 23597 |
| RRID | AB_2221519 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y305 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACOT9 antibody 15901-1-AP | Download protocol |
| IHC protocol for ACOT9 antibody 15901-1-AP | Download protocol |
| WB protocol for ACOT9 antibody 15901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACOT9 Polyclonal antibody (Cat# 15901-1-AP) has 3 citations published in J Transl Med, EMBO Mol Med, and Liver Int. The cited studies span cancer biology (laryngeal squamous cell carcinoma and hepatocellular carcinoma), mitochondrial metabolism, and fatty acid metabolism reprogramming. Applications include WB and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Transl Med ACOT9, a mitochondrial metabolism-related gene, promotes ROS-associated epithelial remodeling in laryngeal squamous cell carcinoma. | ||
EMBO Mol Med A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy | ||
Liver Int Acyl-CoA thioesterase 9 promotes tumor growth and metastasis through reprogramming of fatty acid metabolism in hepatocellular carcinoma
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