Tested Applications
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human colon cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16140-1-AP targets ACSF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9199 Product name: Recombinant human ACSF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-615 aa of BC012053 Sequence: FNGKKALEAISRERGTFLYGTPTMFVDILNQPDFSSYDISTMCGGVIAGSPAPPELIRAIINKINMKDLVVAYGTTENSPVTFAHFPEDTVEQKAESVGRIMPHTEARIMNMEAGTLAKLNTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYWTGDVATMNEQGFCKIVGRSKDMIIRGGENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTVEEIKAFCKGKISHFKIPKYIVFVTNYPLTISGKIQKFKLREQMERHLNL Predict reactive species |
| Full Name | acyl-CoA synthetase family member 2 |
| Calculated Molecular Weight | 615 aa, 68 kDa |
| Observed Molecular Weight | 55-70 kDa |
| GenBank Accession Number | BC012053 |
| Gene Symbol | ACSF2 |
| Gene ID (NCBI) | 80221 |
| RRID | AB_2878222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96CM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACSF2 antibody 16140-1-AP | Download protocol |
| IHC protocol for ACSF2 antibody 16140-1-AP | Download protocol |
| WB protocol for ACSF2 antibody 16140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-ACSF2 antibody (Cat# 16140-1-AP) has 12 citations published in journals including Clinical Cancer Research, Diabetologia, Free Radical Biology and Medicine, Frontiers in Immunology, Redox Report, Life Sciences, Journal of Nutritional Biochemistry, Molecular Carcinogenesis, Kaohsiung Journal of Medical Sciences, Immunological Research, Antioxidants, and Prostaglandins & Other Lipid Mediators. Research fields span ferroptosis, renal injury, diabetes, thyroid carcinoma, cervical cancer, breast cancer, ulcerative colitis, osteosarcoma, osteoarthritis, and lipid metabolism.
| Species | Application | Title |
|---|---|---|
Clin Cancer Res Biomarker identification and risk prediction model development for differentiated thyroid carcinoma lung metastasis based on primary lesion proteomics | ||
Antioxidants (Basel) PET117 Deficiency Confers Ferroptosis Resistance Through ACSF2 Downregulation in Cervical Cancer. | ||
Free Radic Biol Med Inhibition of ACSF2 protects against renal ischemia/reperfusion injury via mediating mitophagy in proximal tubular cells
| ||
Front Immunol Integration of transcriptomics and machine learning for insights into breast cancer: exploring lipid metabolism and immune interactions | ||
Redox Rep ACSF2-PGK1 interaction promotes ferroptosis in renal tubular epithelial cells of diabetic nephropathy by regulating Keap1/Nrf2 signaling |
Reviews
What customers say
One reviewer (Dawei Liu) used this antibody for Western Blot at a 1:1000 dilution on HEK293T cells and rated it 3 out of 5. The antibody worked as intended but produced several non-specific bands near the target band, suggesting room for improved specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Dawei (Verified Customer) (10-02-2025) | Works as intended, however there were quite a few non-specific bands near the actual band
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