Tested Applications
| Positive WB detected in | mouse brain tissue, mouse heart tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25484-1-AP targets ACSF3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21979 Product name: Recombinant human ACSF3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 226-576 aa of BC028399 Sequence: LVHKWAWTKDDVILHVLPLHHVHGVVNALLCPLWVGATCVMMPEFSPQQVWEKFLSSETPRINVFMAVPTIYTKLMEYYDRHFTQPHAQDFLRAVCEEKIRLMVSGSAALPLPVLEKWKNITGHTLLERYGMTEIGMALSGPLTTAMRLPGSVGTPLPGVQVRIVSENPQREACSYTIHAEGDERGTKVTPGFEEKEGELLVRGPSVFREYWNKPEETKSAFTLDGWFKTGDTVVFKDGQYWIRGRTSVDIIKTGGYKVSALEVEWHLLAHPSITDVAVIGVPDMTWGQRVTAVVTLREGHSLSHRELKEWARNVLAPYAVPSELVLVEEIPRNQMGKIDKKALIRHFHPS Predict reactive species |
| Full Name | acyl-CoA synthetase family member 3 |
| Calculated Molecular Weight | 576 aa, 64 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC028399 |
| Gene Symbol | ACSF3 |
| Gene ID (NCBI) | 197322 |
| RRID | AB_2880099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q4G176 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACSF3 antibody 25484-1-AP | Download protocol |
| IHC protocol for ACSF3 antibody 25484-1-AP | Download protocol |
| WB protocol for ACSF3 antibody 25484-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACSF3 Polyclonal antibody (Cat# 25484-1-AP) has 3 total citations. These appear in Science Translational Medicine, Cell Genomics, and Bioconjugate Chemistry. The associated research fields include metabolic disease and protein modification (methylmalonylation in methylmalonic acidemia), human genetics and metabolic homeostasis (ACSF3 variant effects on height and basal metabolic rate), and biochemical biosensing (malonyl-CoA detection at subcellular scales). All three citations used the antibody for Western blot applications.
| Species | Application | Title |
|---|---|---|
Sci Transl Med Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin | ||
Cell Genom An ancient regulatory variant of ACSF3 influences the coevolution of increased human height and basal metabolic rate via metabolic homeostasis | ||
Bioconjug Chem Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.
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