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WB analysis using 22401-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 22401-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22401-1-AP
WB result of ACSL4/FACL4 antibody (22401-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4/FACL4 transfected HeLa cells.
WB analysis using 22401-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 22401-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 22401-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 22401-1-AP
IP result of anti-ACSL4/FACL4 (IP:22401-1-AP, 4ug; Detection:22401-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.
IHC staining of mouse brain using 22401-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ACSL4/FACL4 Polyclonal antibody

Name: ACSL4/FACL4 Polyclonal antibody
Brand: Proteintech
SKU: 22401-1-AP
Rating: 5.00 (1 reviews)

ACSL4/FACL4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 22401-1-AP

Publications(530)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

ACSL4, FACL4, ACS4, ACSL 4, Arachidonate--CoA ligase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (530)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	BV-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, COLO 320 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, RAW 264.7 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 15 publications below
WB	See 467 publications below
IHC	See 78 publications below
IF	See 49 publications below
IP	See 8 publications below
CoIP	See 4 publications below

Product Information

22401-1-AP targets ACSL4/FACL4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, zebrafish, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18085 Product name: Recombinant human ACSL4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 143-236 aa of BC034959 Sequence: LGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMY Predict reactive species
Full Name	acyl-CoA synthetase long-chain family member 4
Calculated Molecular Weight	711 aa, 79 kDa
Observed Molecular Weight	79 kDa, 74 kDa
GenBank Accession Number	BC034959
Gene Symbol	ACSL4
Gene ID (NCBI)	2182
RRID	AB_2832995
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60488
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Protocols

Product Specific Protocols
IHC protocol for ACSL4/FACL4 antibody 22401-1-AP	Download protocol
IP protocol for ACSL4/FACL4 antibody 22401-1-AP	Download protocol
WB protocol for ACSL4/FACL4 antibody 22401-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature 7-Dehydrocholesterol dictates ferroptosis sensitivity Authors - Yaxu Li View Article
human	CoIP	Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression Authors - Shiqiao Qiu View Article
human	WB	Nat Commun URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers Authors - Zhiwen Ding View Article
rat	WB,IHC	Nat Commun Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes Authors - Wenkai Wang View Article
human	WB	Adv Sci (Weinh) Development and Application of the CRISPR-dcas13d-eIF4G Translational Regulatory System to Inhibit Ferroptosis in Calcium Oxalate Crystal-Induced Kidney Injury Authors - Ziqi He View Article
human	WB	Adv Sci (Weinh) Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation Authors - Chen Duan View Article