ACTB Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | WB |
| Species specificity | human, mouse, rat, zebrafish; other species not tested. |
| Cited species | human |
| Positive IHC detected in | human heart |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | IHC: 1:50-1:200 IF: 1:20-1:50 |
IP = Immunoprecipitation
Western blot of Hela cell with anti-Actin-Beta (20536-1-AP) at various dilutions.
Immunohistochemistry of paraffin-embedded human heart using 20536-1-AP(ACTB Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart using 20536-1-AP(ACTB Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ACTB antibody 20536-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | ACTB fusion protein ag14521 | Full name | actin, beta |
|
Calculated molecular weight |
375aa,42kd |
Observed molecular
weight |
42kd |
|
GenBank accession number |
BC002409 | Gene ID (NCBI) | 60 |
| Gene symbol | ACTB | Synonyms | PS1TP5BP1 |
Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha ( ACTC1, cardiac muscle 1), alpha 1 ( ACTA1, skeletal muscle) and 2 ( ACTA2, aortic smooth muscle), beta ( ACTB), gamma 1 ( ACTG1) and 2 ( ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta Actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody was generated by full length beta actin protein and can cross-react with other actins. |
