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ARP3/ARP3B Polyclonal antibody

ARP3/ARP3B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13822-1-AP

Synonyms

Actin like protein 3, Actin related protein 3, ACTR3, ACTR3B, Arp11, ARP3, ARP3/ARP3B, Arp4



Tested Applications

Positive WB detected inA431 cells, mouse spleen tissue, human liver tissue, A549 cells, HL-60 cells, HeLa cells, Jurkat cells, K-562 cells, PC-3 cells
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13822-1-AP targets ARP3/ARP3B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARP3/ARP3B fusion protein Ag4922
Full Name ARP3 actin-related protein 3 homolog (yeast)
Calculated Molecular Weight 418 aa 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC044590
Gene Symbol ACTR3
Gene ID (NCBI) 10096
RRIDAB_2221967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

Protocols

Product Specific Protocols
WB protocol for ARP3/ARP3B antibody 13822-1-APDownload protocol
IHC protocol for ARP3/ARP3B antibody 13822-1-APDownload protocol
IP protocol for ARP3/ARP3B antibody 13822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Actin cables and comet tails organize mitochondrial networks in mitosis.

Authors - Andrew S Moore
ratWB,IF

ACS Nano

Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.

Authors - Yiyuan Kang
mouseIF

PLoS Genet

N-wasp is required for structural integrity of the blood-testis barrier.

Authors - Xiang Xiao
humanIP

EMBO Rep

WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.

Authors - Kailiang Zhao
WB

Elife

Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein

Authors - Congyan Zhang
humanWB

Int J Biol Sci

Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.

Authors - Xin Dong