Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10086-1-AP targets ACVR1B in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0129 Product name: Recombinant human ACVR1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 211-448 aa of BC000254 Sequence: EIIGKGRFGEVWRGRWRGGDVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTIEGMIKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSGGVHEEYQLPYYDLVPSDPSIEEMRKVVC Predict reactive species |
| Full Name | activin A receptor, type IB |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC000254 |
| Gene Symbol | ACVR1B |
| Gene ID (NCBI) | 91 |
| RRID | AB_2877723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36896 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACVR1B antibody 10086-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACVR1B Polyclonal antibody (Cat# 10086-1-AP) has 10 citations spanning journals including Bioactive Materials, Cancer Letters, eBioMedicine, JAHA, Reproductive Biology and Endocrinology, Journal of Ovarian Research, Molecular and Cellular Endocrinology, Animals, PeerJ, and Journal of Toxicological Sciences. Research fields cover bone repair, lung adenocarcinoma ferroptosis, preeclampsia, cardiac remodeling, ovarian cancer, skeletal muscle differentiation, and oxidative stress.
| Species | Application | Title |
|---|---|---|
Bioact Mater Bioprinted constructs that simulate nerve-bone crosstalk to improve microenvironment for bone repair | ||
Cancer Lett The YTHDF3-DT/miR-301a-3p /INHBA axis attenuates autophagy-dependent ferroptosis in lung adenocarcinoma
| ||
EBioMedicine H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia
| ||
J Am Heart Assoc Activin Receptor-Like Kinase 4 Haplodeficiency Mitigates Arrhythmogenic Atrial Remodeling and Vulnerability to Atrial Fibrillation in Cardiac Pathological Hypertrophy. | ||
J Ovarian Res HOXB2 promotes cisplatin resistance by upregulating lncRNA DANCR in ovarian cancer |
