Tested Applications
| Positive WB detected in | mouse brain tissue, human liver tissue, mouse lung tissue, mouse skeletal muscle tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human colon cancer tissue, human liver tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15304-1-AP targets ADAMTSL4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7781 Product name: Recombinant human ADAMTSL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 775-1074 aa of BC027478 Sequence: TQSCQLRLCGHWEVGSPWSQCSVRCGRGQRSRQVRCVGNNGDEVSEQECASGPPQPPSREACDMGPCTTAWFHSDWSSKCSAECGTGIQRRSVVCLGSGAALGPGQGEAGAGTGQSCPTGSRPPDMRACSLGPCERTWRWYTGPWGECSSECGSGTQRRDIICVSKLGTEFNVTSPSNCSHLPRPPALQPCQGQACQDRWFSTPWSPCSRSCQGGTQTREVQCLSTNQTLSTRCPPQLRPSRKRPCNSQPCSQRPDDQCKDSSPHCPLVVQARLCVYPYYTATCCRSCAHVLERSPQDPS Predict reactive species |
| Full Name | ADAMTS-like 4 |
| Calculated Molecular Weight | 117 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC027478 |
| Gene Symbol | ADAMTSL4 |
| Gene ID (NCBI) | 54507 |
| RRID | AB_2222624 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UY14 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ADAMTSL4 antibody 15304-1-AP | Download protocol |
| IHC protocol for ADAMTSL4 antibody 15304-1-AP | Download protocol |
| IP protocol for ADAMTSL4 antibody 15304-1-AP | Download protocol |
| WB protocol for ADAMTSL4 antibody 15304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ADAMTSL4 Polyclonal antibody (Cat# 15304-1-AP) has 4 total citations published in International Journal of Nanomedicine, Matrix Biology, Frontiers in Molecular Biosciences, and Oncotarget. The associated research fields span osteoarthritis treatment via nanomicelles, extracellular matrix biology and lysyl oxidase interactions, esophageal squamous cell carcinoma prognosis, and nasopharyngeal carcinoma metastasis.
| Species | Application | Title |
|---|---|---|
Int J Nanomedicine Pioglitazone-Loaded Cartilage-Targeted Nanomicelles (Pio@C-HA-DOs) for Osteoarthritis Treatment | ||
Matrix Biol Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families. | ||
Front Mol Biosci An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma. | ||
Oncotarget Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications. |
