ADAP1 Polyclonal antibody

ADAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13911-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Cnt a1, Centaurin-alpha-1, Centaurin alpha 1, CENTA1, ArfGAP with dual PH domains 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis of human brain using 13911-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13911-1-AP (ADAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 13911-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13911-1-AP (ADAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 13911-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13911-1-AP (ADAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 13911-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13911-1-AP (ADAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 13911-1-AP
Immunofluorescent analysis of MCF-7 cells, using ADAP1 antibody 13911-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 13911-1-AP
Immunofluorescent analysis of Hela cells, using ADAP1 antibody 13911-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human brain tissue
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13911-1-AP targets ADAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4898 Product name: Recombinant human ADAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-374 aa of BC033747 Sequence: MAKERRRAVLELLQRPGNARCADCGAPDPDWASYTLGVFICLSCSGIHRNIPQVSKVKSVRLDAWEEAQVEFMASHGNDAARARFESKVPSFYYRPTPSDCQLLREQWIRAKYERQEFIYPEKQEPYSAGYREGFLWKRGRDNGQFLSRKFVLTEREGALKYFNRNDAKEPKAVMKIEHLNATFQPAKIGHPHGLQVTYLKDNSTRNIFIYHEDGKEIVDWFNALRAARFHYLQVAFPGASDADLVPKLSRNYLKEGYMEKTGPKQTEGFRKRWFTMDDRRLMYFKDPLDAFARGEVFIGSKESGYTVLHGFPPSTQGHHWPHGITIVTPDRKFLFACETESDQREWVAAFQKAVDRPMLPQEYAVEAHFKHKP Predict reactive species
Full Name	ArfGAP with dual PH domains 1
Calculated Molecular Weight	374 aa, 43 kDa
Observed Molecular Weight	43 kDa
GenBank Accession Number	BC033747
Gene Symbol	ADAP1
Gene ID (NCBI)	11033
RRID	AB_2257889
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75689
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ADAP1 antibody 13911-1-AP	Download protocol
IHC protocol for ADAP1 antibody 13911-1-AP	Download protocol
WB protocol for ADAP1 antibody 13911-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The ADAP1 Polyclonal antibody (Cat# 13911-1-AP) has 2 citations. One appeared in Cancer Letters (IF 11.8, 2024), studying CD24-mediated metastasis and chemoresistance in esophageal squamous cell carcinoma via the Arf6-ERK pathway. The other was published in Apoptosis (IF 9, 2026), investigating lncRNA AC098613.1's role in acute myeloid leukemia cell differentiation through the CDC5L/ADAP1/NRD1 axis. Both citations used the antibody for Western Blot in human samples, spanning oncology and hematological malignancy research fields.

Species	Application	Title
human	WB	Cancer Lett CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma. Authors - Pan Hong View Article
human	WB	Apoptosis LncRNA AC098613.1 promotes acute myeloid leukemia cell differentiation through CDC5L/ADAP1/NRD1 axis. Authors - Qingling Jiang View Article