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ADAR1 Antibody
Rabbit Polyclonal
Catalog number: 14330-1-AP



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Applications
| Tested applications | ELISA, IHC, IF, IP |
| Cited applications | |
| Species specificity | human, mouse, rat, pig; other species not tested. |
| Cited species | |
| Positive IHC detected in | human gliomas |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | IHC: 1:20-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-ADAR (14330-1-AP for IP and Detection) with Y79 cell lysate,
Immunohistochemistry of paraffin-embedded human gliomas using 14330-1-AP(ADAR1 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human gliomas using 14330-1-AP(ADAR1 Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ADAR antibody 14330-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | ADAR1 fusion protein ag5609 | Full name | adenosine deaminase, RNA-specific |
|
Calculated molecular weight |
136kd |
Observed molecular
weight |
|
|
GenBank accession number |
BC038227 | Gene ID (NCBI) | 103 |
| Gene symbol | ADAR | Synonyms | ADAR1; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88dsRBP; p136 |
Background
ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an interferon (IFN) inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH). This antibody is specific to ADAR1. |
Related Primary Antibodies:
Related Fusion Proteins:
Related Secondary Antibodies:
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