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Beta Adducin Polyclonal antibody

Beta Adducin Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14640-1-AP

Synonyms

ADD2, ADDB, adducin 2 (beta), Beta adducin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Adducin Polyclonal antibody (14640-1-AP)

    WB analysis using 14640-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14640-1-AP (Beta adducin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using Beta Adducin Polyclonal antibody (14640-1-AP)

    IP experiment of K-562 using 14640-1-AP

    IP result of anti-Beta Adducin (IP:14640-1-AP, 4ug; Detection:14640-1-AP 1:1000) with K-562 cells lysate 3200ug.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

14640-1-AP targets Beta Adducin in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Adducin fusion protein Ag6223
Full Name adducin 2 (beta)
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC065525
Gene Symbol ADD2
Gene ID (NCBI) 119
RRIDAB_2223568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Beta Adducin antibody 14640-1-APDownload protocol
IP protocol for Beta Adducin antibody 14640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Adv

Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment.

Authors - Hong Ouyang
View Article