Beta Adducin Polyclonal antibody

Beta Adducin Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14640-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ADD2, ADD 2, Beta-adducin, Adducin2, adducin 2 (beta)

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIPIHC

WB analysis using 14640-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14640-1-AP (Beta adducin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of K-562 using 14640-1-AP
IP result of anti-Beta Adducin (IP:14640-1-AP, 4ug; Detection:14640-1-AP 1:1000) with K-562 cells lysate 3200ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 14640-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14640-1-AP (Beta Adducin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

14640-1-AP targets Beta Adducin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6223 Product name: Recombinant human ADD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC065525 Sequence: MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLIL Predict reactive species
Full Name	adducin 2 (beta)
Calculated Molecular Weight	81 kDa
Observed Molecular Weight	110 kDa
GenBank Accession Number	BC065525
Gene Symbol	Beta Adducin
Gene ID (NCBI)	119
RRID	AB_2223568
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35612
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for Beta Adducin antibody 14640-1-AP	Download protocol
IP protocol for Beta Adducin antibody 14640-1-AP	Download protocol
WB protocol for Beta Adducin antibody 14640-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Science Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells Authors - Zhi Lin View Article
mouse	WB	Cell Rep Pyruvate dehydrogenase complex E1 subunit α crotonylation modulates cocaine-associated memory through hippocampal neuron activation Authors - Hongchun Li View Article
human	IF	Sci Adv Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment. Authors - Hong Ouyang View Article