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Tested Applications
| Positive WB detected in | NIH/3T3 cells, K-562 cells, mouse kidney tissue |
| Positive IP detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
17585-1-AP targets Gamma Adducin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11318 Product name: Recombinant human ADD3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 364-707 aa of BC062559 Sequence: WKVGEIEFEGLMRTLDNLGYRTGYAYRHPLIREKPRHKSDVEIPATVTAFSFEDDTVPLSPLKYMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDSSKVSGGTPIKIEDPNQFVPLNTNPNEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMQFEDDDHGPPAPPNPFSHLTEGELEEYKRTIERKQQGLEDAEQELLSDDASSVSQIQSQTQSPQNVPEKLEENHELFSKSFISMEVPVMVVNGKDDMHDVEDELAKRVSRLSTSTTIENIEITIKSPEKIEEVLSPEGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA Predict reactive species |
| Full Name | adducin 3 (gamma) |
| Calculated Molecular Weight | 76 kDa, 79 kDa |
| Observed Molecular Weight | 80 kDa, 90 kDa |
| GenBank Accession Number | BC062559 |
| Gene Symbol | Gamma Adducin |
| Gene ID (NCBI) | 120 |
| RRID | AB_2223579 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UEY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for Gamma Adducin antibody 17585-1-AP | Download protocol |
| WB protocol for Gamma Adducin antibody 17585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Mol Cell Biol QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer. | ||
Stem Cell Res Ther Knockdown of long noncoding RNA SAN rejuvenates aged adipose-derived stem cells via miR-143-3p/ADD3 axis
| ||
PLoS One Dysferlin and other non-red cell proteins accumulate in the red cell membrane of diamond-blackfan anemia patients. | ||
Cell Genom Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders |
