ADK Polyclonal antibody

ADK Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15477-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Adenosine 5 phosphotransferase, adenosine kinase, ADK, AK

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis using 15477-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15477-1-AP (ADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 15477-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15477-1-AP (ADK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 15477-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15477-1-AP (ADK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HeLa cells, HepG2 cells, PC-3 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

15477-1-AP targets ADK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7783 Product name: Recombinant human ADK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC003568 Sequence: MTSVRENILFGMGNPLLDISAVVDKDFLDKYSLKPNDQILAEDKHKELFDELVKKFKVEYHAGGSTQNSIKVAQWMIQQPHKAATFFGCIGIDKFGEILKRKAAEAHVDAHYYEQNEQPTGTCAACITGDNRSLIANLAAANCYKKEKHLDLEKNWMLVEKARVCYIAGFFLTVSPESVLKVAHHASENNRIFTLNLSAPFFSQFYKESLMKVMPYVDILFGNETEAATFAREQGFETKDIKEIAKKTQALPKMNSKRQRIVIFTQGRDDTIMATESEVTAFAVLDQDQKEIIDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASIIIRRTGCTFPEKPDFH Predict reactive species
Full Name	adenosine kinase
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	40-46 kDa
GenBank Accession Number	BC003568
Gene Symbol	ADK
Gene ID (NCBI)	132
RRID	AB_2222085
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P55263
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for ADK antibody 15477-1-AP	Download protocol
WB protocol for ADK antibody 15477-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse	WB	Neurochem Res NLRP3 Inflammasome Activation Enhances ADK Expression to Accelerate Epilepsy in Mice Authors - Haiju Zhang View Article
mouse	WB	Cardiovasc Drugs Ther Class Effect of SGLT2 Inhibitors Against Doxorubicin-Induced Cardiotoxicity via Regulating Adenosine Kinase Mediated-Cardiac Oxidative Stress. Authors - Jueqian Yan KD Validated View Article