Tested Applications
| Positive WB detected in | DU 145 cells, mouse brain tissue, rat brain tissue, HeLa cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21093-1-AP targets AFAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15346 Product name: Recombinant human AFAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 382-730 aa of BC032777 Sequence: DLKTHIVSIPLRGCEVIPGLDCKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNGYAHPSGTALHYDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKRTGSNAAQYKYGKNRVEADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKPQAILEEKLKQLEEECRQKEAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAVLKKSQAAPGSSPCRGHVLRKAKEWELKNGT Predict reactive species |
| Full Name | actin filament associated protein 1 |
| Calculated Molecular Weight | 730 aa, 81 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC032777 |
| Gene Symbol | AFAP1 |
| Gene ID (NCBI) | 60312 |
| RRID | AB_10732810 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N556 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Actin filament-associated protein 1 (AFAP1) encodes a motor fiber-associated protein, which organizes a network for linking other proteins, including SRC proto-oncogene, non-receptor tyrosine kinase (Src) and protein kinase C . A long non-coding RNA, namely AFAP1-antisense RNA 1 (AS1), is transcribed from the antisense strand of this gene and potentially regulates its expression.(PMID: 31086663, PMID: 35193469)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AFAP1 antibody 21093-1-AP | Download protocol |
| IHC protocol for AFAP1 antibody 21093-1-AP | Download protocol |
| WB protocol for AFAP1 antibody 21093-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AFAP1 Polyclonal antibody (Cat# 21093-1-AP) has 7 citations published in Nucleic Acids Research, J Exp Clin Cancer Res, Int J Mol Med, Biol Res, Mol Biol Cell, Oncotarget, and Chonnam Med J. Research fields span antiviral signaling, osteosarcoma EMT, myocardial ischemia-reperfusion injury, colorectal cancer, cytoskeletal protein organization, cholangiocarcinoma metastasis, and bladder cancer migration.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling
| ||
J Exp Clin Cancer Res LncRNA AFAP1-AS1 promotes tumorigenesis and epithelial-mesenchymal transition of osteosarcoma through RhoC/ROCK1/p38MAPK/Twist1 signaling pathway. | ||
Int J Mol Med Curculigoside attenuates myocardial ischemia‑reperfusion injury by inhibiting the opening of the mitochondrial permeability transition pore. | ||
Biol Res Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer. | ||
Mol Biol Cell Spatial proximity of proteins surrounding zyxin under force-bearing conditions.
| ||
Reviews
What customers say
One reviewer (Joleen C.) rated this antibody 5/5 stars, using it at a 1:1,000 dilution on MDCK GII cells for Western blotting. They reported excellent performance with strong signal visible at just 5 seconds of exposure and noted that the typical dilution worked well without needing optimization. Overall, the feedback is highly positive, highlighting reliable and sensitive detection in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joleen (Verified Customer) (05-04-2019) | Works excellently for WB. Strong signal by 5s exposure and with typical dilution.
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