AFF4 Antibody
Rabbit Polyclonal
Catalog number: 14662-1-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | L02 cells, HeLa cells, HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
L02 cells were subjected to SDS PAGE followed by western blot with 14662-1-AP(AFF4 Antibody) at dilution of 1:500
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | AFF4 fusion protein ag6336 | Full name | AF4/FMR2 family, member 4 |
|
Calculated molecular weight |
127kd |
Observed molecular
weight |
140kd |
|
GenBank accession number |
BC063007 | Gene ID (NCBI) | 27125 |
| Gene symbol | AFF4 | Synonyms | AF5Q31; MCEF; MGC75036 |
Background
Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929]. |
