AFG3L2 Antibody
Rabbit Polyclonal
Catalog number: 14631-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells, HEK-293 cells, human brain tissue, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human kidney |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 14631-1-AP(AFG3L2 Antibody) at dilution of 1:1200
Immunohistochemistry of paraffin-embedded human kidney using 14631-1-AP(AFG3L2 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney using 14631-1-AP(AFG3L2 Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | AFG3L2 fusion protein ag6209 | Full name | AFG3 ATPase family gene 3-like 2 (yeast) |
|
Calculated molecular weight |
88kd |
Observed molecular
weight |
80kd |
|
GenBank accession number |
BC065016 | Gene ID (NCBI) | 10939 |
| Gene symbol | AFG3L2 | Synonyms | FLJ25993 |
Background
AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5). This antibody is specific to AFG3L2. |
