Tested Applications
| Positive WB detected in | mouse liver tissue, L02 cells |
| Positive IHC detected in | human lung cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
16990-1-AP targets AGBL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10592 Product name: Recombinant human AGBL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 272-621 aa of BC030651 Sequence: QDGRHYFSLTWTFQFPHNKDTCYFAHCYPYTYTNLQEYLSGINNDPVRSKFCKIRVLCHTLARNMVYILTITTPLKNSDSRKRKAVILTARVHPGETNSSWIMKGFLDYILGNSSDAQLLRDTFVFKVVPMLNPDGVIVGNYRCSLAGRDLNRNYTSLLKESFPSVWYTRNMVHRLMEKREVILYCDLHGHSRKENIFMYGCDGSDRSKTLYLQQRIFPLMLSKNCPDKFSFSACKFNVQKSKEGTGRVVMWKMGIRNSFTMEATFCGSTLGNKRGTHFSTKDLESMGYHFCDSLLDYCDPDRTKYYRCLKELEEMERHITLEKVFEDSDTPVIDITLDVESRELTFLLR Predict reactive species |
| Full Name | ATP/GTP binding protein-like 3 |
| Calculated Molecular Weight | 73 kDa, 116 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC030651 |
| Gene Symbol | AGBL3 |
| Gene ID (NCBI) | 340351 |
| RRID | AB_2878337 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AGBL3, also named as CCP3, is a metallocarboxypeptidase that may play a role in the processing of tubulin. AGBL3 has some isoforms with MW 107 kDA, 73 kDa, 116 kDa and 20 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AGBL3 antibody 16990-1-AP | Download protocol |
| WB protocol for AGBL3 antibody 16990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
