AGFG1 Polyclonal antibody

AGFG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12670-1-AP

Synonyms

AGFG1, ArfGAP with FG repeats 1, HIV 1 Rev binding protein, HRB, Nucleoporin like protein RIP, RAB, Rev interacting protein, RIP



Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, HepG2 cells, HT-1080 cells, Jurkat cells, K-562 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12670-1-AP targets AGFG1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGFG1 fusion protein Ag3352
Full Name ArfGAP with FG repeats 1
Calculated Molecular Weight 562 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC030592
Gene Symbol AGFG1
Gene ID (NCBI) 3267
RRIDAB_2258077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AGFG1 antibody 12670-1-APDownload protocol
IHC protocol for AGFG1 antibody 12670-1-APDownload protocol
IP protocol for AGFG1 antibody 12670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell

A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.

Authors - Peng Dai

Proc Natl Acad Sci U S A

Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.

Authors - Yoshinori Kanemori