Tested Applications
| Positive WB detected in | SKOV-3 cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11967-1-AP targets AGR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2577 Product name: Recombinant human AGR3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC058284 Sequence: MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEEGLFYAQKSKKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNLMHETTDKNLSPDGQYVPRIMFVDPSLTVRADIAGRYSNRLYTYEPRDLPLLIENMKKALRLIQSEL Predict reactive species |
| Full Name | anterior gradient homolog 3 (Xenopus laevis) |
| Calculated Molecular Weight | 166 aa, 19 kDa |
| Observed Molecular Weight | 19-25 kDa |
| GenBank Accession Number | BC058284 |
| Gene Symbol | AGR3 |
| Gene ID (NCBI) | 155465 |
| RRID | AB_2877809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TD06 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AGR3 antibody 11967-1-AP | Download protocol |
| IHC protocol for AGR3 antibody 11967-1-AP | Download protocol |
| WB protocol for AGR3 antibody 11967-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AGR3 Polyclonal antibody (Cat# 11967-1-AP) has 6 total citations published in Cell, J Transl Med, Front Pharmacol, Oncotarget, iScience, and Elife. Research fields include spatial proteomics, breast cancer angiogenesis, COPD airway epithelium, ovarian cancer progression, and auditory hair cell development in chicken. Applications cited span WB, IHC, and IF across human, mouse, and chicken species.
| Species | Application | Title |
|---|---|---|
Cell High-resolution spatially resolved proteomics of complex tissues based on microfluidics and transfer learning | ||
J Transl Med Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers | ||
Front Pharmacol AGR3 Regulates Airway Epithelial Junctions in Patients with Frequent Exacerbations of COPD. | ||
Oncotarget Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer. | ||
iScience Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium | ||
