AHCY Antibody
Mouse Monoclonal
Catalog number: 66019-1-Ig
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | MCF7 cells |
| Positive IHC detected in | human testis |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
MCF7 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig(AHCY Antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human testis using 66019-1-Ig(AHCY Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis using 66019-1-Ig(AHCY Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using AHCY antibody 66019-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Mouse | Purification method | Protein A purification |
| Isotype | IgG2b | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | AHCY fusion protein ag18036 | Full name | S-adenosylhomocysteine hydrolase |
|
Calculated molecular weight |
48kd |
Observed molecular
weight |
48kd |
|
GenBank accession number |
BC011606 | Gene ID (NCBI) | 191 |
| Gene symbol | AHCY | Synonyms | SAHH |
Background
AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).This antibody is specific to AHCY. |
