Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cell |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 6 publications below |
Product Information
22045-1-AP targets AHI1 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16885 Product name: Recombinant human AHI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC094800 Sequence: MPTAESEAKVKTKVRFEELLKTHSDLMREKKKLKKKLVRSEENISPDTIRSNLHYMKETTSDDPDTIRSNLPHIKETTSDDVSAANTNNLKKSTRVTKNKLRNTQLATENPNGDASVEEDKQGKPNKKVIKTVPQLTTQDLKPETPENKVDSTHQKTHTKPQPGVDHQKSEKANEGREETDLEEDEELMQAYQCHVTEEMAKEIKRKIRKKLKEQLTYFPSDTLFHDDKLSSEKRKKKKEVPVFSKAETSTLTISGDTVEGEQKKESSVRSVSSDSHQDDEISSMEQSTEDSMQDDTKPKPKKTKKKTKAVADNNEDVDGDGVHEITSRDSPVYPKCLLDDDLVLGVYIH Predict reactive species |
| Full Name | Abelson helper integration site 1 |
| Calculated Molecular Weight | 1196 aa, 137 kDa |
| Observed Molecular Weight | 137-150 kDa |
| GenBank Accession Number | BC094800 |
| Gene Symbol | AHI1 |
| Gene ID (NCBI) | 54806 |
| RRID | AB_11182927 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N157 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AHI1, encoding the Jouberin protein, is an alternatively spliced signaling molecule that contains seven Trp-Asp (WD) repeats, an SH3 domain, and numerous SH3-binding sites. AHI1 is expressed strongly in embryonic hindbrain and forebrain. AHI1 is involved in vesicle trafficking and is required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. AHI1, as a positive modulator of classical Wnt signaling, may play a key role in ciliary signaling during cerebellar embryonic development (PMID: 15467982, PMID: 21623382).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AHI1 antibody 22045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling. | ||
Front Cell Dev Biol Superresolution Microscopy Reveals Distinct Phosphoinositide Subdomains Within the Cilia Transition Zone. | ||
Mol Biol Cell Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function.
| ||
Sci Rep Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts. | ||
FEBS Open Bio Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins | ||
bioRxiv Ciliopathy-associated protein, CEP290, is required for ciliary necklace and outer segment membrane formation in retinal photoreceptors |
