Tested Applications
| Positive WB detected in | mouse kidney tissue, HeLa cells, rat liver tissue, HepG2 cells, PC-12 ceslls |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20886-1-AP targets AIFM2/ FSP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13412 Product name: Recombinant human AIFM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-373 aa of BC023601 Sequence: MGSQVSVESGALHVVIVGGGFGGIAAASQLQALNVPFMLVDMKDSFHHNVAALRASVETGFAKKTFISYSVTFKDNFRQGLVVGIDLKNQMVLLQGGEALPFSHLILATGSTGPFPGKFNEVSSQQAAIQAYEDMVRQVQRSRFIVVVGGGSAGVEMAAEIKTEYPEKEVTLIHSQVALADKELLPSVRQEVKEILLRKGVQLLLSERVSNLEELPLNEYREYIKVQTDKGTEVATNLVILCTGIKINSSAYRKAFESRLASSGALRVNEHLQVEGHSNVYAIGDCADVRTPKMAYLAGLHANIAVANIVNSVKQRPLQAYKPGALTFLLSMGRNDGVGQISGFYVGRLMVRLTKSRDLFVSTSWKTMRQSPP Predict reactive species |
| Full Name | apoptosis-inducing factor, mitochondrion-associated, 2 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC023601 |
| Gene Symbol | AIFM2/ FSP1 |
| Gene ID (NCBI) | 84883 |
| RRID | AB_2878756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis-associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis-Inducing Factor), a mitochondrion-associated enzyme that relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AIFM2/ FSP1 antibody 20886-1-AP | Download protocol |
| IHC protocol for AIFM2/ FSP1 antibody 20886-1-AP | Download protocol |
| IP protocol for AIFM2/ FSP1 antibody 20886-1-AP | Download protocol |
| WB protocol for AIFM2/ FSP1 antibody 20886-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-FSP1 antibody (Cat# 20886-1-AP) has 307 total citations. Publications span top-tier journals including Nature, Science, Cell, Nature Communications, Nature Cell Biology, Cell Death & Disease, Autophagy, Redox Biology, and Molecular Cell, among many others. Key research fields include ferroptosis biology, oncology, neurodegeneration, liver fibrosis, acute kidney injury, cardiovascular disease, immunology, environmental toxicology, and cellular metabolism.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
All four reviewers gave this antibody a perfect 5-star rating for Western Blot applications. Users consistently praised its high specificity and reliable performance. One researcher highlighted its value in studying AIFM2/FSP1 expression related to ferroptosis in melanoma cell lines. Another noted it solved their long-standing difficulty in detecting this protein, calling the results "perfect." The antibody was used at a 1:1000 dilution across melanoma cells and cancer cell lines, with all reviewers reporting successful detection without issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Casey (Verified Customer) (11-30-2025) | Worked well
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Haley (Verified Customer) (11-26-2025) | Highly specific
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Jelan (Verified Customer) (10-31-2025) | For the protein AIFM2/FSP1, it itself is an inhibition of FSP1 sensitizes cancer cells to ferroptosis and suppresses tumor growth and it is associated with cell death. we use it in our western blots to see if our cell lysates from our experiment to see if they over or under express ferroptosis.
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Selin (Verified Customer) (03-14-2024) | We've been having problems to identify this protein, but luckily this antibody worked perfectly.
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