Tested Applications
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12510-2-AP targets AK5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3203 Product name: Recombinant human AK5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-328 aa of BC012467 Sequence: PDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGELLREELASESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEEFGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKINAEGTPEDVFLQLCTAIDSIF Predict reactive species |
| Full Name | adenylate kinase 5 |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 63 kDa |
| GenBank Accession Number | BC012467 |
| Gene Symbol | AK5 |
| Gene ID (NCBI) | 26289 |
| RRID | AB_2305376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6K8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AK5 antibody 12510-2-AP | Download protocol |
| IHC protocol for AK5 antibody 12510-2-AP | Download protocol |
| WB protocol for AK5 antibody 12510-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AK5 Polyclonal antibody (Cat# 12510-2-AP) has 6 citations published in Nature Communications, Phytomedicine, iScience, Molecular Brain, Molecular Biology Reports, and Journal of Neurological Sciences. Research fields span perinatal brain development, cognitive function and neural plasticity, breast cancer progression, Alzheimer's disease, oligodendrocyte apoptosis, and temporal lobe epilepsy.
| Species | Application | Title |
|---|---|---|
Nat Commun Perinatal brain developmental transition revealed by transcriptomic and proteomic analyses of Bama miniature pigs. | ||
Phytomedicine 20(S)-protopanaxadiol targets brain-enriched adenylate kinase 5 to improve cognitive function via regulating hippocampal neural plasticity. | ||
iScience AK5 suppresses breast cancer progression and modulates anti-PD-L1 efficacy via the miR-182-5p/PD-L1 axis.
| ||
Mol Brain Adenylate kinase 5, a novel genetic risk factor for Alzheimer's disease, regulates microglial inflammatory activation.
| ||
Mol Biol Rep Single-cell sequencing reveals that AK5 inhibits apoptosis in AD oligodendrocytes by regulating the AMPK signaling pathway | ||
J Neurol Sci Down-regulation of adenylate kinase 5 in temporal lobe epilepsy patients and rat model. |
Reviews
What customers say
All four reviewers rated this antibody positively (4–5 stars) for Western Blot applications. Users consistently report clear, specific bands at the expected ~60 kDa size, with one reviewer noting reliable performance across multiple melanoma cell lines at a 1:1000 dilution. Overall feedback highlights consistency and ease of use for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kristen (Verified Customer) (12-12-2025) | very consistent band (~60kDa) across multiple melanoma cell lines
|
Casey (Verified Customer) (12-01-2025) | Clear results
|
Haley (Verified Customer) (11-26-2025) | Great for western blot
|
Diana (Verified Customer) (11-03-2025) |
|
