AKAP10 Polyclonal antibody

AKAP10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12356-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

AKAP 10, AKAP10, D AKAP 2, D AKAP2, PRKA10

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 12356-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12356-1-AP (AKAP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 12356-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12356-1-AP (AKAP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 12356-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 12356-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lymphoma using 12356-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lymphoma using 12356-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12356-1-AP (AKAP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected in	human colon cancer tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12356-1-AP targets AKAP10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3020 Product name: Recombinant human AKAP10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 314-663 aa of BC017055 Sequence: IPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGSSDSSASQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKGSMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDIMQQAQYDQPLEKSTKL Predict reactive species
Full Name	A kinase (PRKA) anchor protein 10
Calculated Molecular Weight	662 aa, 74 kDa
Observed Molecular Weight	74 kDa
GenBank Accession Number	BC017055
Gene Symbol	AKAP10
Gene ID (NCBI)	11216
RRID	AB_2225588
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43572
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for AKAP10 antibody 12356-1-AP	Download protocol
WB protocol for AKAP10 antibody 12356-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The AKAP10 Polyclonal antibody (Cat# 12356-1-AP) has 1 citation. It was cited in Journal of Medicinal Chemistry (IF 7.3) in a 2022 study on discovering a small-molecule inhibitor that disrupts TRBP-Dicer interaction to modulate microRNA biogenesis against hepatocellular carcinoma. The research field spans cancer biology (liver cancer), RNA regulation, and drug discovery. The antibody was used in Western Blot for human samples.

Species	Application	Title
human	WB	J Med Chem Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis. Authors - Ting Peng View Article